Year: 2013
Type: other
Abstract: The great potential of mass spectrometry (MS)-based proteomics as a quantitative application in clinical chemistry has been explored and shown since more than a decade.As a result of maturation of strategies as well as MS-equipment, the field of quantitative proteomics now experiences a growing interest for implementation in the clinic.MS methods have the potential to outperform the currently applied clinical immunoassays and offer multiple opportunities to support standardization initiatives for the measurement of protein biomarkers in routine clinical practice.For successful introduction and broad use of MS-based quantitative protein assays in clinical chemistry, several important concepts for quality assurance generally accepted in the clinical chemistry community have to be borne in mind.To translate the quality specifications of routine clinical assays to newly developed MS-based methods, common knowledge of the underlying principles to define minimal, desirable and optimal analytical performance, such as those based on biological variation, is essential.In this review critical steps in the complete quantitative clinical chemistry proteomics (qCCP) workflow that may contribute to the total variation of the MS-procedure are outlined, in particular for applications that involve liquid chromatography in combination with stable-isotope dilution methodology and multiple reaction monitoring MS (LC-SID-MRM-MS).Furthermore, in this review well-known clinical chemistry concepts of metrological traceability and commutability of reference materials are introduced with regard to MS strategies, and the potential of qCCP in standardization of clinical protein assays is emphasized.Critical steps for safeguarding analytical performance at the pre-, and analytical phase,
Source: Translational Proteomics
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $1,950
OpenAlex ID: W4210970776