Work

Tools of the trade: plasmid repositories and standardized plasmid manipulation for molecular and synthetic biology

Year: 2022

Type: article

Abstract: Plasmids are extrachromosomal genetic elements capable of autonomous replication within a host cell. They play a key role in bacterial ecology and evolution, facilitating the mobilization of accessory genes by horizontal gene transfer. Crucially, plasmids also serve as valuable tools in modern molecular biology. Here, we highlight recent articles aimed at implementing standardized plasmid assembly techniques and plasmid repositories to promote open science as well as to improve experimental reproducibility across laboratories. Research focused on assisting these fundamental aims is a further step towards improving standardization in molecular and synthetic biology.

Source: Microbial Biotechnology

DOI: 10.1111/1751-7915.14012

Authors: Stephen K. Dolan, Miguel A. Matilla

Institutions: Georgia Institute of Technology, Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,200

OpenAlex ID: W4211002940

FWCI: 0.123

Cites: 22

Cited by: 1

Related to: 10