Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization
Year: 2022
Type: article
Abstract: model for studying normal and pathologic human brain development. Here, we optimized existing protocols to streamline the generation of forebrain COs from hiPSCs. We employ these COs to define the impact of disease-causing mutations on cell fate, differentiation, maturation, and morphology relevant to neurodevelopmental disorders. Although limited to forebrain CO identity, this schema requires minimal external interference and is amenable to low-throughput biochemical assays. For complete details on the use and execution of this profile, please refer to Anastasaki et al. (2020) and Wegscheid et al. (2021).
Source: STAR Protocols
Institution: Washington University in St. Louis
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
Awards: G1234843147, G1632423083, G3017522143, G304139194, Defining the Mechanistic Basis for Neurofibromatosis-1 Nervous System Disease Heterogeneity, G4446621599, G770300975, National Research Service Award-Medical Scientist, Training in Regenerative Medicine, Developing Human Induced Pluripotent Stem Cell Precision Oncology Models
OpenAlex ID: W4211021275