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Thawing of mouse embryonic fibroblasts (MEFs) for hPSC cultures v1

Year: 2022

Type: preprint

Abstract: This protocol describes the thawing of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture. General notes 1. Throughout this protocol, the term hPSC is used to collectively refer to both hiPSCs and hESCs. All described procedures have been tested and work equally well for hiPSCs and hESCs. 2. MEFs were obtained as described in Manipulating the Mouse Embryo: A Laboratory Manual, Third Edition (ISBN: 0879695919)

DOI: 10.17504/protocols.io.b4n4qvgw

Authors: Hanqin Li, Oriol Busquets, Steven Poser, Dirk Hockemeyer, Frank Soldner

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4211053417

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