Work

Carbohydrate microarrays

Year: 2012

Type: review

Abstract: In the last decade, carbohydrate microarrays have been core technologies for analyzing carbohydrate-mediated recognition events in a high-throughput fashion. A number of methods have been exploited for immobilizing glycans on the solid surface in a microarray format. This microarray-based technology has been widely employed for rapid analysis of the glycan binding properties of lectins and antibodies, the quantitative measurements of glycan-protein interactions, detection of cells and pathogens, identification of disease-related anti-glycan antibodies for diagnosis, and fast assessment of substrate specificities of glycosyltransferases. This review covers the construction of carbohydrate microarrays, detection methods of carbohydrate microarrays and their applications in biological and biomedical research.

Source: Chemical Society Reviews

DOI: 10.1039/c2cs35401b

Authors: Sungjin Park, Jeffrey C. Gildersleeve, Ola Blixt, Injae Shin

Institutions: Yonsei University, Frederick National Laboratory for Cancer Research, National Cancer Institute, Center for Cancer Research, University of Copenhagen

Language: English

Topic: Glycosylation and Glycoproteins Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute

Award: Development of a Carbohydrate Microarray