Cupriavidus metallidurans CH34 Possesses Aromatic Catabolic Versatility and Degrades Benzene in the Presence of Mercury and Cadmium
Year: 2022
Type: article
Abstract: Heavy metal co-contamination in crude oil-polluted environments may inhibit microbial bioremediation of hydrocarbons. The model heavy metal-resistant bacterium Cupriavidus metallidurans CH34 possesses cadmium and mercury resistance, as well as genes related to the catabolism of hazardous BTEX aromatic hydrocarbons. The aims of this study were to analyze the aromatic catabolic potential of C. metallidurans CH34 and to determine the functionality of the predicted benzene catabolic pathway and the influence of cadmium and mercury on benzene degradation. Three chromosome-encoded bacterial multicomponent monooxygenases (BMMs) are involved in benzene catabolic pathways. Growth assessment, intermediates identification, and gene expression analysis indicate the functionality of the benzene catabolic pathway. Strain CH34 degraded benzene via phenol and 2-hydroxymuconic semialdehyde. Transcriptional analyses revealed a transition from the expression of catechol 2,3-dioxygenase (tomB) in the early exponential phase to catechol 1,2-dioxygenase (catA1 and catA2) in the late exponential phase. The minimum inhibitory concentration to Hg (II) and Cd (II) was significantly lower in the presence of benzene, demonstrating the effect of co-contamination on bacterial growth. Notably, this study showed that C. metallidurans CH34 degraded benzene in the presence of Hg (II) or Cd (II).
Source: Microorganisms
Language: English
Subfield: Pollution
Field: Environmental Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,303
Awards: GENOME-GUIDED METABOLIC AND ADAPTIVE STRESS-RESPONSE CHARACTERIZATION OF CHILEAN NATIVE AND MODEL BACTERIA AND THEIR APPLICATIONS IN HYDROCARBON BIOREMEDIATION UNDER ABIOTIC STRESS, G1916470654, CATABOLISM OF AROMATICS COMPOUNDS BY CUPRIAVIDUS METALLIDURANS STRAINS AND THEIR BIOTECHNOLOGICAL APPLICATIONSIN POLLUTED SOILS, G4814518166, G5068280523, G8177253822
OpenAlex ID: W4213046793