Year: 2018
Type: article
Abstract: Next-generation sequencing is an efficient method for mapping whole genomes.However, this approach falls short when trying to sequence DNA damage.Wu, McKeague and Sturla developed a new method, 'Click-Code-Seq', which can be used to sequence 8-oxoG at single nucleotide resolution within whole genomes.Their approach utilizes repair enzymes that specifically replace the damaged site with an artificial nucleotide, followed by click chemistry to introduce a short oligonucleotide suitable for nextgeneration sequencing.Using this approach, distinct patterns of oxidative damage in a variety of different genomic elements have been discovered.
Authors: Morten O. Loehr, Aaron Johnson, Ashkan Karimi, Nathan W. Luedtke
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4213174198