Faculty Opinions recommendation of BMP signaling stimulates cellular differentiation at multiple steps during cartilage development.
Year: 2005
Type: peer-review
Abstract: Mice.Col2-Gal4 mice have been described (24).UAS-caBmpr1A embryos and mice were genotyped by PCR by using primer 5Ј-ACG CTT GCC AAG ATG GTT GA-3Ј and a primer for the LacZ tag sequence contained in pWEXP3C, 5Ј-CGG TCA GAC GAT TCA TTG GCA CCAT-3Ј.Bmpr1b-null mice have been described (6). Skeletal Preparation and Histology.Alizarin red and͞or alcian blue staining was modified after McLeod's method (27).Carcasses were fixed in 95% ethanol, stained with alcian blue and͞or alizarin red, cleared in 1% KOH, and kept in 50% glycerol.Embryos were fixed in 4% paraformaldehyde͞PBS at 4°C overnight, dissected, processed, and embedded in paraffin.Sections were cut with 5-m thickness, subjected to hematoxylin͞eosin staining, in situ hybridization, and immunostaining.In Situ Hybridization.In situ hybridization on paraffin sections with radiolabeled antisense RNA probes was performed on paraformaldehyde-fixed tissues as described (28).Probes for Col10a1 Conflict of interest statement: No conflicts declared.
Author: Brendan Lee
Institution: Baylor College of Medicine
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funder: National Institutes of Health
OpenAlex ID: W4214548936