Work

Faculty Opinions recommendation of TET1 and hydroxymethylcytosine in transcription and DNA methylation fidelity.

Year: 2011

Type: peer-review

Abstract: Enzymes catalysing the methylation of the 5-position of cytosine (mC) have essential roles in regulating gene expression and maintaining cellular identity.Recently, TET1 was found to hydroxylate the methyl group of mC, converting it to 5-hydroxymethyl cytosine (hmC).Here we show that TET1 binds throughout the genome of embryonic stem cells, with the majority of binding sites located at transcription start sites (TSSs) of CpG-rich promoters and within genes.The hmC modification is found in gene bodies and in contrast to mC is also enriched at CpG-rich TSSs.We provide evidence further that TET1 has a role in transcriptional repression.TET1 binds a significant proportion of Polycomb group target genes.Furthermore, TET1 associates and colocalizes with the SIN3A co-repressor complex.We propose that TET1 fine-tunes transcription, opposes aberrant DNA methylation at CpG-rich sequences and thereby contributes to the regulation of DNA methylation fidelity.

Source: Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature

DOI: 10.3410/f.9949956.10673054

Authors: Hongjun Song, Junjie Guo

Institutions: Johns Hopkins University, Johns Hopkins Medicine, Whitehead Institute for Biomedical Research

Language: English

Topic: Epigenetics and DNA Methylation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Kræftens Bekæmpelse, Wellcome Trust, National Research Foundation, Danmarks Grundforskningsfond

OpenAlex ID: W4214727966

Cites: 30

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Related to: 10