Work

Identification and Functional Characterization of Tyrosine Decarboxylase from Rehmannia glutinosa

Year: 2022

Type: article

Abstract: Rehmannia glutinosa is an important medicinal plant that has long been used in Chinese traditional medicine. Acteoside, one of the bioactive components from R. glutinosa, possessed various pharmacological activities for human health; however, the molecular mechanism of acteoside formation is not fully understood. In the current study, a novel tyrosine decarboxylase (designated as RgTyDC2) was identified from the R. glutinosa transcriptome. Biochemical analysis of RgTyDC2 showed RgTyDC2 uses tyrosine and dopa as the substrate to produce tyramine and dopamine, respectively, and it displays higher catalytic efficiency toward tyrosine than dopa. Moreover, the transcript level of RgTyDC2 was consistent with the accumulation pattern of acteoside in R. glutinosa, supporting its possible role in the biosynthesis of acteoside in vivo.

Source: Molecules

DOI: 10.3390/molecules27051634

Authors: Yuanjun Li, Yanan Wang, Longyu Huang, Chunrong Chen, Na An, Xiaoke Zheng

Institutions: Henan University of Chinese Medicine, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Henan University

Language: English

Topic: Plant Gene Expression Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,408

Funder: China Agricultural University