TRIM26 inhibits gastric cancer development by regulating SLC7A11-mediated ferroptosis pathway
Year: 2022
Type: preprint
Abstract: Background TRIM26 is an E3 ubiquitin ligase that has been shown to regulate tumor progression. However, the role of TRIM26 in gastric cancer (GC) remains unclear. The purpose of this study is to investigate the role and mechanism of TRIM26 in GC. Methods CCK-8 assay, wound healing assay and Transwell invasion assay were used to explore the specific role of TRIM26 in GC cells. Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect the expression levels of TRIM26, SLC7A11, COX-2, Glutathione peroxidase 4 (GPX4) and other related genes in cells. The levels of GSH, MDA and ROS were detected by kits. Results The expression of TRIM26 was down-regulated in GC tissues and cells. Low expression of TRIM26 enhanced the viability, migration activity and invasive ability of MGC-803 cells. Overexpression of TRIM26 significantly reduced cell viability, migration and invasion abilities. Further studies found that TRIM26 participated in the process of ferroptosis by regulating the expression of SLC7A11, thereby affecting the development of GC. Conclusion We demonstrated for the first time that TRIM26 inhibits the proliferation, migration and invasion of GC cells by regulating SLC7A11-mediated ferroptosis. TRIM26 may be a new target for the clinical treatment of GC.
Source: Research Square
Authors: Xueyan Guo, Yulong Li, Bingbing Wan, Yifei Lv, Xue Wang, Guisheng Liu, Ping Wang
Institution: Shaanxi Provincial People's Hospital
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4220675429