High-Frequency Direct Organogenesis from Cotyledonary Node Explants and Plantlet Regeneration of Peanut (Arachis hypogaea) Cultivars
Year: 2021
Type: article
Abstract: The efficient plantlets regeneration of peanut is the most important and a primary step to successfully transform gene and apply recently developed genome editing techniques for crop yield improvement.The purpose of this research is to develop protocol for peanut cultivars applying different concentration of hormones for selected peanut cultivars and develop plantlets regeneration protocol.There is no previously reported protocol for the Chinese peanut cultivar N3 and Yu-hua-14.We optimized shoot and root regeneration protocol for two peanut cultivars.Both cultivars showed positive response for the cytokinin plant growth hormone 6-benzylaminopurine (BAP) and thidiazuron (TDZ).The highest shooting rate (97%) was found in a medium supplemented with 4 mg/L BAP and (94.33%) for 1 mg/L TDZ.Hence, more shoot initiation was observed at higher concentration of BAP as compared to TDZ.However, the maximum root regeneration (81%) was found on medium containing 0.3 mg/L 2, 4-dichlorophenoxyacetic acid (2,4-D) and the highest rooting rate (96.33%) was found in a medium containing 1 mg/L α-naphthalene acetic acid (NAA), indicating lower concentration of NAA induce more rooting rate than 2,4-D treatment.In this study, cotyledonary node was used, and this method was found to be efficient and rapid for in vitro peanut regeneration.
Authors: Abraham Lamboro, Xiao Han, Songnan Yang, Xueying Li, Dan Yao, Abdourazak Alio Moussa, Mazhar Rasul Chaudhry, Harold Ilboudo, Baixing Song, Qiulin Wu, Yongyi Xing, Jun Zhang
Institutions: Jilin Agricultural University, Northeast Normal University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W4220732379