Work

Characterization of a large gated SPAD array for in vivo fluorescence lifetime imaging of drug target engagement

Year: 2022

Type: conference-paper

Abstract: Near-Infrared wide-field Fluorescence Lifetime Imaging (FLI) has become an increasingly popular method due to its unique specificity in sensing the cellular micro-environment and/or protein-protein interactions via FRET, but the approach is still challenging due to inefficient detection modules. Here, we report on the characterization of a large gated SPAD array, SwissSPAD2, towards in vivo preclinical imaging of FLI-FRET. Fluorescence decay fitting as well as phasor analysis are used to demonstrate the ability of SwissSPAD2 to accurately quantify short lifetimes and associated lifetime parameters in both in vitro and in vivo experiments, in full agreement with gated ICCD measurements.

DOI: 10.1117/12.2609888

Authors: Jason T. Smith, Alena Rudkouskaya, Shan Gao, Arin Ulku, Claudio E. Bruschini, Edoardo Charbon, Shimon Weiss, Margarida Barroso, Xavier R. Intes, Xavier Michalet

Institutions: Albany Medical College, Rensselaer Polytechnic Institute, École Polytechnique Fédérale de Lausanne, University of California, Los Angeles

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W4220828876

FWCI:

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