ADAD2 regulates heterochromatin in meiotic and post-meiotic male germ cells via translation of MDC1
Year: 2022
Type: article
Abstract: ABSTRACT Male germ cells establish a unique heterochromatin domain, the XY-body, early in meiosis. How this domain is maintained through the end of meiosis and into post-meiotic germ cell differentiation is poorly understood. ADAD2 is a late meiotic male germ cell-specific RNA-binding protein, loss of which leads to post-meiotic germ cell defects. Analysis of ribosome association in Adad2 mouse mutants revealed defective translation of Mdc1, a key regulator of XY-body formation, late in meiosis. As a result, Adad2 mutants show normal establishment but failed maintenance of the XY-body. Observed XY-body defects are concurrent with abnormal autosomal heterochromatin and ultimately lead to severely perturbed post-meiotic germ cell heterochromatin and cell death. These findings highlight the requirement of ADAD2 for Mdc1 translation, the role of MDC1 in maintaining meiotic male germ cell heterochromatin and the importance of late meiotic heterochromatin for normal post-meiotic germ cell differentiation.
Source: Journal of Cell Science
DOI: 10.1242/jcs.259196
Institution: Rutgers, The State University of New Jersey
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Awards: Male Germ Cell RNA Binding Proteins and Nuclear RNAs in Male Fertility, G1687640479, G3568840548, Male Germ Cell RNA Binding Proteins and Nuclear RNAs in Male Fertility, G4267653156, RNA Editing in the Neonatal and Adult Testis, High Throughput Production and Cryopreservation of Knockout Mice, G738160294, G8722142496
OpenAlex ID: W4220882330