An evidence appraisal of heart organoids in a dish and commensurability to human heart development in vivo
Year: 2022
Type: review
Abstract: Stem-cell derived in vitro cardiac models have provided profound insights into mechanisms in cardiac development and disease. Efficient differentiation of specific cardiac cell types from human pluripotent stem cells using a three-step Wnt signaling modulation has been one of the major discoveries that has enabled personalized cardiovascular disease modeling approaches. Generation of cardiac cell types follow key development stages during embryogenesis, they intuitively are excellent models to study cardiac tissue patterning in primitive cardiac structures. Here, we provide a brief overview of protocols that have laid the foundation for derivation of stem-cell derived three-dimensional cardiac models. Further this article highlights features and utility of the models to distinguish the advantages and trade-offs in modeling embryonic development and disease processes. Finally, we discuss the challenges in improving robustness in the current models and utilizing developmental principles to bring higher physiological relevance. In vitro human cardiac models are complimentary tools that allow mechanistic interrogation in a reductionist way. The unique advantage of utilizing patient specific stem cells and continued improvements in generating reliable organoid mimics of the heart will boost predictive power of these tools in basic and translational research.
Source: BMC Cardiovascular Disorders
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,690
Awards: Modeling Endothelial Dysfunction in LMNA-related Dilated Cardiomyopathy, The Role of Genomic Ancestry in Mediating Breast and Prostate Cancer Therapy Cardiotoxicity (Basic Science Project), G7136137331, Unraveling Adverse Effects of Checkpoint Inhibitors Using iPSC-derived Cardiac Organoids, G8657864569, Stanford Medicine Center for Longevity and Healthy Aging, Deciphering the Endothelial Cell-Cardiomyocyte Crosstalk in LMNA Cardiomyopathy, Unraveling the role of endothelium in chemotherapy-induced cardiotoxicity, Stanford Alzheimer's Disease Research Center
OpenAlex ID: W4220927683