Year: 2022
Type: preprint
Abstract: Background Terpenoids are economically and ecologically important compounds and vital constituents of rose flower fragrance and rose essential oil. Terpene synthase (TPS) is a primary enzyme for terpenoid biosynthesis. However, only a few TPS genes have been characterized in rose, owing to the lack of a systematic TPS gene analysis. Additionally, the key gene RcNUDX1 was analyzed because of the presence of unique geraniol pathways in rose. Results The present study identified 74 putative RcTPS genes through a comprehensive bioinformatics analysis; of these genes, 49 genes were predicted to encode functional TPS enzymes. The TPS-a subfamily had the largest members, and tandem duplication was the main mechanism for driving TPS gene family expansion. The collinearity analysis with the genome of other Rosaceae plants and grape indicated that four RcTPS genes may be derived from the dicotyledonous plant ancestors. During the flower opening process of butterfly rose (Rosa chinensis ‘Mutabilis’, MU), RcTPS genes exhibited two main expression trends, namely high and low, in old and fresh petals. Similarly, dark treatment decreased the expression of four RcTPSs and increased the expression of three RcTPSs in the MU petals. Most of the monoterpenes were released more from old MU petals than from fresh MU petals, which may be related to RcLIN-NERS3 and RcNUDX1-1a4 genes. Analysis of volatile emission, gene expression, sequence structure, and collinearity indicated that RcTPS18 may encode (E,E)-α-farnesene synthase. The three genes whose expression levels increased at the late stage and after dark treatment exhibited similar expression patterns with some senescence marker genes, which indicated that these genes might be involved in petal senescence or cell death. Conclusions The present study revealed the differential RcTPS gene expression patterns in rose petals. These findings may be useful for elucidating the molecular mechanism of terpenoid metabolism in rose and are vital for future studies on terpene regulation.
Source: Research Square
Authors: Ying Kong, Huan Wang, Lixin Lang, Xiaoying Dou, Jinrong Bai
Institution: Beijing Academy of Science and Technology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: G1831907904, G3746695221
OpenAlex ID: W4223961041