Year: 2022
Type: article
Study design: Observational Study
Abstract: The isolation of Streptococcus pneumoniae serotypes in systemic tissues of patients with invasive disease versus the nasopharynx of healthy individuals with asymptomatic carriage varies widely. Some serotypes are hyper-invasive, particularly serotype 1, but the underlying genetics remain poorly understood due to the rarity of carriage isolates, reducing the power of comparison with invasive isolates. Here, we use a well-controlled genome-wide association study to search for genetic variation associated with invasiveness of serotype 1 pneumococci from a serotype 1 endemic setting in Africa. We found no consensus evidence that certain genomic variation is overrepresented among isolates from patients with invasive disease than asymptomatic carriage. Overall, the genomic variation explained negligible phenotypic variability, suggesting a minimal effect on the disease status. Furthermore, changes in lineage distribution were seen with lineages replacing each other over time, highlighting the importance of continued pathogen surveillance. Our findings suggest that the hyper-invasiveness is an intrinsic property of the serotype 1 strains, not specific for a "disease-associated" subpopulation disproportionately harboring unique genomic variation.
Source: Genome Biology and Evolution
DOI: 10.1093/gbe/evac052
Authors: Chrispin Chaguza, Chinelo Ebruke, Madikay Senghore, Stephanie W. Lo, Peggy-Estelle Tientcheu, Rebecca A. Gladstone, Gerry Tonkin-Hill, Jennifer E. Cornick, Marie Yang, Archibald Worwui, Lesley McGee, Robert F. Breiman, Keith P. Klugman, Aras Kadioglu, Dean B. Everett, Grant Mackenzie, Nicholas J. Croucher, Anna Roca, Brenda A. Kwambana-Adams, Martin Antonio, Stephen D. Bentley
Institutions: University of Liverpool, University of Cambridge, Wellcome Sanger Institute, MRC Unit the Gambia, Medical Research Council, Harvard University, University of Oslo, Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Centers for Disease Control and Prevention, Emory University, Khalifa University of Science and Technology, London School of Hygiene & Tropical Medicine, Murdoch Children's Research Institute, Imperial College London, University College London, University of Warwick
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,714
Awards: G1247782102, G1274357658, G1690586642, Prevention and Restriction of Antimicrobial Resistance in Pneumococci by Multi-Level Modelling, G4300644782, G6144121480, G6520098237, G7093086574, Wellcome Trust Sanger Institute - generic account for deposition of all core- funded research papers., G7624493998, University of Liverpool - to enable and drive the development of improved and appropriate vaccines against invasive pneumococcal disease for use in high burden low income co..., G988044597
OpenAlex ID: W4224223696