Two targets that play a role in the immune microenvironment of breast cancer
Year: 2022
Type: preprint
Abstract: Breast cancer (BRCA) is a malignancy that occurs in breast epithelium or ductal epithelium. The progression of BRCA is greatly influenced by the tumor microenvironment (TME). Studies have shown that Pachymic acid (PA) had an inhibitory effect on the proliferation and metastasis of BRCA cells and could also enhance immunity. We planned to use the methods of network pharmacology and bio-information analysis to clarify the role of this molecule in the TME of BRCA and its target. We predicted the potential targets of PA. In addition, by comparing the cancer profiles in the The Tumor Genome Atlas (TCGA) genome with normal profiles, differentially expressed genes (DEGs) were obtained. At the intersection of predicted targets and DEGs were 16 important genes. Enrichment analysis and survival analysis were performed on these genes. Two hub genes were found in combination with the RNA-seq expression and protein expression in T cell subsets by Immunohistochemical analysis. Finally, we used the GSE42568 data to construct a risk prediction model and obtained independent prognostic indicators. And we identified two PA targets that played a role in the TME, thus providing new ideas for research on BRCA.
Source: Research Square
Authors: Li-Ning Wang, Ya-Qing Zhou, Yue-Xuan Li, Qing Zhang, Lu Wang
Institution: Tianjin University of Traditional Chinese Medicine
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
Funder: Tianjin University
OpenAlex ID: W4224250442