Whole genome sequencing of cyanobacterium Nostoc sp. CCCryo 231-06 using microfluidic single cell technology
Year: 2022
Type: article
Abstract: The Nostoc sp. strain CCCryo 231-06 is a cyanobacterial strain capable of surviving under extreme conditions and thus is of great interest for the astrobiology community. The knowledge of its complete genome sequence would serve as a guide for further studies. However, a major concern has been placed on the effects of contamination on the quality of sequencing data without a reference genome. Here, we report the use of microfluidic technology combined with single cell sequencing and de novo assembly to minimize the contamination and recover the complete genome of the Nostoc strain CCCryo 231-06 with high quality. 100% of the whole genome was recovered with all contaminants removed and a strongly supported phylogenetic tree. The data reported can be useful for comparative genomics for phylogenetic and taxonomic studies. The method used in this work can be applied to studies that require high-quality assemblies of genomes of unknown microorganisms.
Source: iScience
Institutions: Mayo Clinic, Leibniz Institute of Freshwater Ecology and Inland Fisheries, GFZ Helmholtz Centre for Geosciences, Technische Universität Berlin, Washington State University, Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR), University of Edinburgh, Fraunhofer Institute for Cell Therapy and Immunology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,000
Funders: Mayo Clinic, California Department of Fish and Game, Mayo Foundation for Medical Education and Research, Engineering Research Centers, European Space Agency, Deutsche Forschungsgemeinschaft, Deutsches Zentrum für Luft- und Raumfahrt, National Institutes of Health, Science and Technology Facilities Council, National Center for Advancing Translational Sciences, Division of Loan Repayment, H2020 European Research Council
OpenAlex ID: W4224311987