Year: 2022
Type: article
Abstract: Recent literature demonstrates that platinum-based chemotherapeutic drugs in physiological solvents display higher efficacy in destabilizing cancer cells.As human cancer cells come in over 200 different varieties, it would be beneficial to test the efficacy of these drugs using a wider spectrum of cells.Utilizing the well-tested HeLa cervical cancer cells as a control for the effects of these drugs, we assessed the impact of platinum-based cisplatin, carboplatin, and oxaliplatin on ML-1 thyroid cancer cells.Through the XTT Viability assay, we found that ML-1 cells are more resistant to cisplatin and oxaliplatin with an IC 50 value at least four times higher than those for the same drugs in HeLa cells.It has been consistently shown that the oxidative stress caused by these chemicals was more pronounced in HeLa cells than in ML-1 cells, but the only measurable results were found 24 hours after treatment.We also show that a high percentage of HeLa cells displayed apoptosis with even 20 µM of these chemicals, which is directly comparable in effect to the 100 µM of chemicals in ML-1 cells.Upon comparing the expression levels of pro-apoptotic enzymes in HeLa and ML-1 cells, we observed that when treated with 40 µM of these chemicals, the levels of pro-apoptotic enzymes were statistically higher for HeLa cells than for ML-1 cells.Our research will provide new insight into the different capacities of each cell line and the treatment regimen for cancer patients in the future.
Source: ACTA SCIENTIFIC MICROBIOLOGY
Authors: Daniel S Kim, Min Zhang, Nhi Le, Seth Harris, Kyoungtae Kim
Institutions: Emory University, Missouri State University
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4225270125