Work

Studying Tunicata WBR Using Botrylloides anceps

Year: 2022

Type: book-chapter

Abstract: Tunicates are marine filter-feeding invertebrates that can be found worldwide and which are the closest phylogenetic group to the vertebrates (Craniata). Of particular interest, colonial tunicates are the only known chordates that can undergo Whole-Body Regeneration (WBR) via vascular budding. In Botrylloides anceps, a fully functional adult regenerates from a fragment of the vascular system in around 2 weeks after amputation. In this chapter, we present protocols to collect B. anceps colonies, confirm their species, breed them in the lab, monitor WBR and perform histological staining on cryosections.

Source: Methods in molecular biology

DOI: 10.1007/978-1-0716-2172-1_16

Authors: Arzu Karahan, Esra Öztürk, Berivan Temiz, Simon Blanchoud

Institutions: Middle East Technical University, University of Fribourg

Language: English

Topic: Planarian Biology and Electrostimulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: Orta Doğu Teknik Üniversitesi, Université de Fribourg

Award: G992181563