Work

Enhancement of Cryo-EM maps by a multiscale tubular filter

Year: 2021

Type: article

Abstract: We present an approach to enhance cryo-electron microscopy (cryo-EM) postprocessed maps based on a multiscale tubular filter. The method determines a tubularness measure locally by the analysis of the eigenvalues of the Hessian matrix. This information is used to enhance elongated local structures and to attenuate blob-like and plate-like structures. The approach, thus, introduces a priori information in the reconstructions to improve their interpretability and analysis at high-resolution. The proposed method has been tested with simulated and real cryo-EM maps including recent reconstructions of the SARS-CoV-2. Our results show that our methods can improve obtained reconstructions.

Source: Optics Express

DOI: 10.1364/oe.444675

Authors: Javier Vargas, José A. Gómez-Pedrero, J. Antonio Quiroga, José Alonso

Institution: Universidad Complutense de Madrid

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,270

Funders: Ministerio de Ciencia, Innovación y Universidades, Ministerio de Ciencia e Innovación