Three-dimensional virtual histology of the cerebral cortex based on phase-contrast X-ray tomography
Year: 2021
Type: article
Abstract: In this work, we optimize the setups and experimental parameters of X-ray phase-contrast computed-tomography for the three-dimensional imaging of the cyto- and myeloarchitecture of cerebral cortex, including both human and murine tissue. We present examples for different optical configurations using state-of-the art synchrotron instruments for holographic tomography, as well as compact laboratory setups for phase-contrast tomography in the direct contrast (edge-enhancement) regime. Apart from unstained and paraffin-embedded tissue, we tested hydrated tissue, as well as heavy metal stained and resin-embedded tissue using two different protocols. Further, we show that the image quality achieved allows to assess the neuropathology of multiple sclerosis in a biopsy sample collected during surgery.
Source: Biomedical Optics Express
DOI: 10.1364/boe.434885
Authors: Marina Eckermann, Franziska van der Meer, Peter Cloetens, Torben Ruhwedel, Wiebke Möbius, Christine Stadelmann, Tim Salditt
Language: English
Subfield: Radiation
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,913
OpenAlex ID: W3209691890