Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria
Year: 2022
Type: article
Abstract: Mosquito vectors are a tremendous public health threat. One in six diseases worldwide is vector-borne transmitted mainly by mosquitoes. In the last couple of years, there have been active Yellow fever virus (YFV) outbreaks in many settings in Nigeria, and nationwide, entomological surveillance has been a significant effort geared towards understanding these outbreaks. In this study, we used a metagenomic sequencing approach to characterize viruses present in vector samples collected during various outbreaks of Yellow fever (YF) in Nigeria between 2017 and 2020. Mosquito samples were grouped into pools of 1 to 50 mosquitoes, each based on species, sex and location. Twenty-five pools of Aedes spp and one pool of Anopheles spp collected from nine states were sequenced and metagenomic analysis was carried out. We identified a wide diversity of viruses belonging to various families in this sample set. Seven different viruses detected included: Fako virus, Phasi Charoen-like virus, Verdadero virus, Chaq like-virus, Aedes aegypti totivirus, cell fusing agent virus and Tesano Aedes virus. Although there are no reports of these viruses being pathogenic, they are an understudied group in the same families and closely related to known pathogenic arboviruses. Our study highlights the power of next generation sequencing in identifying Insect specific viruses (ISVs), and provide insight into mosquito vectors virome in Nigeria.
Source: Scientific Reports
Authors: Judith U. Oguzie, Udoka C. Nwangwu, Paul E. Oluniyi, Testimony J. Olumade, Uwem E. George, Akano Kazeem, Bolajoko E. Bankole, Farida O. Brimmo, Chukwuemeka C. Asadu, Okechukwu C. Chukwuekezie, Josephine C. Ochu, Catherine O. Makwe, Festus A. Dogunro, Cosmas O. Onwude, William E. Nwachukwu, Ebuka K. Ezihe, Gilkenny K. Okonkwo, Ndubuisi E. Umazi, Jacob Maikere, Nneka O. Agashi, Emelda I. Eloy, Stephen O. Anokwu, Angela I. Okoronkwo, Ebuka M. Nwosu, Sandra O. Etiki, Ifeoma M. Ngwu, Chikwe Ihekweazu, Onikepe A. Folarin, Isaac O. O. Komolafe, Christian T. Happi
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,190
Awards: G1461798421, G3390425409, Lassa outbreak response: early intervention and community response capacity in Ondo, Edo and Ebonyi states, Nigeria, G4456492296, G4878468429, G4904316071, Lassa outbreak response: early intervention and community response capacity in Ondo, Edo and Ebonyi states, Nigeria, Genomic Characterization and Surveillance of Microbial Threats in West Africa, G7389463554, G8164097130, Host and Microbial Genetic Determinants of Febrile Illness in West Africa, G963736066
OpenAlex ID: W4229448107