Work

A Genetically Encoded Boronate‐Containing Amino Acid

Year: 2008

Type: article

Abstract: A biological boronate: An orthogonal tRNA/aminoacyl-tRNA synthetase pair has been evolved for the genetic incorporation of a boronic acid into proteins. This amino acid has been used to purify proteins in a one-step scarless purification procedure as well as for the site-specific labeling of proteins using various boronic acid chemistries.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.200803240

Authors: Eric Brustad, Mark L. Bushey, Jae Wook Lee, Dan Groff, Wenshe Liu, Peter G. Schultz

Institutions: Scripps Research Institute, Texas A&M University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: G792181733