Year: 2016
Type: article
Abstract: Comprehensive delineation of complex cellular networks requires high-throughput interrogation of genetic interactions. To address this challenge, we describe the development of a multiplex combinatorial strategy to assess pairwise genetic interactions using CRISPR-Cas9 genome editing and next-generation sequencing. We characterize the performance of combinatorial genome editing and analysis using different promoter and gRNA designs and identified regions of the chimeric RNA that are compatible with next-generation sequencing preparation and quantification. This approach is an important step towards elucidating genetic networks relevant to human diseases and the development of more efficient Cas9-based therapeutics.
Source: PLoS ONE
Institution: Columbia University Irving Medical Center
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,382
Awards: G1220657860, National Center: Multiscale Analysis of Genomic and Cellular Networks (MAGNet), G1760589713, G1761060845, A minimally invasive synthetic bio-driven approach for natural products discovery, G214439962, G2518502058, Storage System for High Performance Computing, G2647030984, G2704988860, G273449253, G3571539437, G3624902447, Centers for Cancer Systems Therapeutics (CaST), G4025963258, G4230373143, G4252360166, High-performance compute cluster for biomedical computing, Computer Studies of Protein Structure and Function, G5520316217, G5850534, G5950577451, CAREER: A Systems Approach to Study Horizontal Acquisition of Regulatory DNA, G6206737223, G7419289890, Functional Metagenomic Reprogramming of the Human Microbiome through Mobilome Eng, G7665417115, G7832175178, G8132658730, G8238768891, G8610538666, Quantitative studies of influenza evolution, G8876996369, G9000022848, G9000175094
OpenAlex ID: W4230852093