Work

Callus induction and proliferation fromRosa hybridaleaf explants

Year: 2015

Type: article

Abstract: To establish a resourceful tissue culture system to induce anthocyanin pigments, research was conducted to obtain high quality callus from leaf explant of Rosa hybrida cv. Pusa Ajay. Of the different treatments employed for callus induction using Murashige and Skoog (MS) medium, a treatment combination of 19.60 μM indole3-butyric acid (IBA) + 4.65 μM kinetin (Kin) + 108.6 mM adenine sulphate (AdS) gave the maximum induction coefficient (97.75 ± 1.11%) along with maximum gain in callus biomass accumulation. This treatment also resulted in the lowest fresh-and dry-cell weight ratio (26.52) suggesting actual gain in callus biomass accumulation. Correlation analysis indicated a significant positive correlation between callus induction coefficient and cell fresh-(r = 0.939) and dry-(r = 0.951) weight.

Source: Indian Journal of Horticulture

DOI: 10.5958/0974-0112.2015.00089.4

Authors: Mohan Ram, K.V. Prasad, T. Janakiram, S.K. Singh, Ajay Arora

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

OpenAlex ID: W4230898377

FWCI:

Cites:

Cited by: 3

Related to: 10