Cloning and expression of the putative gene coding for GTP cyclohydrolase I from Escherichia coli
Year: 1990
Type: article
Abstract: The putative gene coding for GTP cyclohydrolase I of Escherichia coli was isolated from a lambda gt11 expression vector library by using antibodies as a probe and has been subcloned on a 3.8 kb Bam HI fragment in the plasmid vector pUC13. E. coli cells carrying the recombinant plasmid designated pCYH express 100-fold increased levels of the enzyme. The protein formed under the control of the plasmid appears electrophoretically and immunochemically identical with the wild type enzyme.
Source: FEMS Microbiology Letters
Authors: Gerd Katzenmeier, Cornelia Schmid, Adelbert Bacher
Institution: Technical University of Munich
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2044119551