Faculty Opinions recommendation of The fate of human Langerhans cells in hematopoietic stem cell transplantation.
Year: 2006
Type: peer-review
Abstract: Langerhans cells (LC) and other antigen-presenting cells are believed to be critical in initiating graft versus host responses that infl uence the outcome of allogeneic hematopoietic stem cell transplantation.However, their fate in humans is poorly understood.We have sought to defi ne the effect of conditioning regimes and graft versus host disease (GVHD) on the survival of recipient LC and reconstitution of donor cells after transplant.Confocal microscopy of epidermal sheets shows that full intensity transplant (FIT) depletes LC more rapidly than reduced intensity transplant (RIT) at day 0, although the nadir is similar in both at 14-21 d.Recovery occurs rapidly within 40 d in the absence of acute GVHD, but is delayed beyond 100 d when GVHD is active.LC chimerism was determined in sexmismatched transplants using a two-step Giemsa/fl uorescence in situ hybridization assay on isolated cells.Acquisition of donor chimerism at 40 d is more rapid after FIT (97%) than RIT (36.5%), irrespective of blood myeloid engraftment.At 100 d, all transplants achieve at least 90% LC donor chimerism and over half achieve 100%.Complete donor chimerism is associated with prior acute cutaneous GVHD, suggesting a role for allogeneic T cells in promoting LC engraftment.
Author: Nelson Chao
Institution: Duke Medical Center
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funder: European Commission
OpenAlex ID: W4232567392