Work

Detection of fos Protein during Osteogenesis by Monoclonal Antibodies

Year: 1988

Type: article

Abstract: We have generated monoclonal antibodies by using a synthetic peptide corresponding to amino acid positions 4 to 17 of the human fos protein. The antibodies detected both v- and c-fos proteins by immunoprecipitation, immunoblotting, and indirect immunofluorescence. The monoclonal antibodies not only identified the fos protein complex with the cellular 39-kilodalton protein, but also recognized the modified forms of the mouse, rat, and human fos proteins. In day-17 rat embryos, nuclear-staining fos protein could be identified in the cartilage by immunohistochemical staining.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.8.5.2251

Authors: P De Togni, H Niman, V Raymond, P Sawchenko, I M Verma

Institution: Salk Institute for Biological Studies

Language: English

Topic: Bone Metabolism and Diseases

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Cancer Institute