Work

Split DNA Enzyme for Visual Single Nucleotide Polymorphism Typing

Year: 2008

Type: article

Abstract: A probe that changes solution color in the presence of only one of the two DNA sequences, which differ by a single nucleotide, is reported. The probe consists of two oligodeoxyribonucleotides, which form a hydrogen peroxidase-like DNA enzyme when hybridized to the abutting fragments of the complementary analyte. The active peroxidase catalyzes oxidation of colorless substrates to the colored products. The probe allows visual detection of a mutation in Alzheimer's disease related DNA.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja711192e

Author: Dmitry M. Kolpashchikov

Institutions: Columbia University Irving Medical Center, Columbia University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Human Genome Research Institute

Award: Nucleic acid analysis using deoxyribozyme technology