Work

Multicolor adeno-associate virus labeling and 3D digital tracing of enteric plexus in mouse proximal colon v1

Year: 2020

Type: preprint

Abstract: Using a multicolor adeno-associate virus system to label the colonic enteric nervous system for digital tracing of individual neurons and nerve fibers in microcircuits in three-dimensions (3D). The methods include viral vectors retro-orbital injection in mice, preparation of colon tissues, microscopy and 3D digital tracing.

DOI: 10.17504/protocols.io.bqavmse6

Authors: Lixin Wang, Collin Challis, Honghui Liang, Songlin Li, Charless Fowlkes, Aidan Sullivan, Kumar SR, Yvette Taché

Institutions: University of California, Los Angeles, University of California, Irvine, California Institute of Technology

Language: English

Topic: Gastrointestinal motility and disorders

Subfield: Gastroenterology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W4235233054

Cites:

Cited by: 1

Related to: 10