Year: 2008
Type: article
Abstract: In Abbildung 1 b dieser Zuschrift ist die Lage des Signals für nichtmodifiziertes Myoglobin fälschlicherweise mit m/z 16 692 angegeben. Die Abbildung mit dem korrekten Wert m/z 16 962 ist hier gezeigt. Site-selective protein modification with PLP. a) Myoglobin was treated with 2 c at 37 °C for 24 h. After removal of small molecules by gel filtration, oxime 14 was formed after reaction with 13. b) ESI-LCMS studies indicated that only one modification had occurred. c) UV/Vis spectra of 12 subjected to the reaction conditions in the absence (dotted red) and presence (dashed blue) of 2 c. d) Product 14 was digested with trypsin, and the resulting fragments were analyzed by MALDI-TOF MS. The only modified fragment observed contained the N-terminal residue. e) Predicted (left) and observed (right) isotope patterns for the modified myoglobin peptide fragment shown in (d). All MS values agree with those predicted within 0.01 %.
Source: Angewandte Chemie
Authors: Joshua M. Gilmore, Rebecca A. Scheck, Aaron P. Esser‐Kahn, Neel S. Joshi, Matthew B. Francis
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W4235394576