Year: 2014
Type: article
Abstract: Next-generation DNA sequencing has revolutionized genomic studies and is driving the implementation of precision diagnostics. The ability of these technologies to disentangle sequence heterogeneity, however, is limited by their relatively high error rates. A Several single molecule barcoding strategies have been propose to reduce the overall error frequency. A Duplex Sequencing additionally exploits the fact that DNA is double-strand, with one strand reciprocally encoding the sequence information of its complement, and can eliminate nearly all sequencing errors by comparing the sequence of individually tagged amplicons derived from one strand of DNA with that of its complementary strand. This method reduces errors to fewer than one per ten million nucleotides sequenced.
Authors: Edward J Fox, Kate S Reid-Bayliss
Institution: University of Washington
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
Awards: Clonally Expanded Mutations Identify Cancer Precursors in Chronic Inflammation, Fidelity of DNA Synthesis, Genetic Approaches to Aging, Mitochondrial Protective Interventions, Aging and Healthspan, Validation and Advanced Development of Duplex Sequencing, University of Washington Medical Scientist Training Program, Molecular Determinants of Cancer Therapeutic Response, Mutator Phenotype in Human Cancers, Nathan Shock Centers of Excellence in Basic Biology of Aging (P30 Clinical Trial Not Allowed)
OpenAlex ID: W4236190976