Work

Replication Forms of the Gene-Sized DNA Molecules of Hypotrichous Ciliates

Year: 1983

Type: article

Abstract: Using a method for obtaining DNA from 10 to 40 macronuclei for electron microscopy, we analyzed the structure of gene-sized, linear DNA molecules from S-phase macronuclei of two hypotrichous ciliates, Euplotes eurystomus and Styx sp. Three types of putative replicating intermediates were observed: (i) molecules with a bubble close to one end, (ii) molecules with single forks, and (iii) molecules with two forks. We conclude that: (i) each macronuclear DNA molecule replicates as an independent unit, (ii) the molecules contain an origin of replication close to one or both ends, and (iii) the mode of replication is bidirectional.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.3.9.1562

Authors: K G Murti, D M Prescott

Institutions: St. Jude Children's Research Hospital, University of Colorado Boulder

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: St. Jude Children's Research Hospital, American Lebanese Syrian Associated Charities, National Institutes of Health

Awards: G865138149, G9000086855