Construction and Behavior of Circularly Permuted and Telocentric Chromosomes in Saccharomyces cerevisiae
Year: 1986
Type: article
Abstract: We developed techniques that allow us to construct novel variants of Saccharomyces cerevisiae chromosomes. These modified chromosomes have precisely determined structures. A metacentric derivative of chromosome III which lacks the telomere-associated X and Y' elements, which are found at the telomeres of most yeast chromosomes, behaves normally in both mitosis and meiosis. We made a circularly permuted telocentric version of yeast chromosome III whose closest telomere was 33 kilobases from the centromere. This telocentric chromosome was lost at a frequency of 1.6 X 10(-5) per cell compared with a frequency of 4.0 X 10(-6) for the natural metacentric version of chromosome III. An extremely telocentric chromosome whose closet telomere was only 3.5 kilobases from the centromere was lost at a frequency of 6.0 X 10(-5). The mitotic stability of telocentric chromosomes shows that the very high frequency of nondisjunction observed for short linear artificial chromosomes is not due to inadequate centromere-telomere separation.
Source: Molecular and Cellular Biology
DOI: 10.1128/mcb.6.9.3166
Authors: A W Murray, J W Szostak
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Award: G5847689315
OpenAlex ID: W1822293882