Year: 2005
Type: other
Abstract: Abrasion: A scraping away of a portion of the surface of the cartilage to stimulate a repair response from the underlying bone.i Aggrecan: A typical proteoglycan found in cartilage tissue.Albumin secretion: Important function of hepatocytes in vivo and one of several surrogate markers for hepatocyte function in vitro.In vivo, albumin binds molecules and drugs in the blood and also contributes to the maintenance of plasma volume and blood osmotic pressure. Alginate:A linear copolymer of β-D-mannuronic acid and α-L-guluronic acid that forms a 3-dimensional gel when exposed to divalent or trivalent cations.The gel can be used to entrap cells and can be dissolved with chelating agents, such as sodium citrate.Alginate bead: A small (∼10 µl) polymerized sphere of alginate gel, possibly containing cells, formed by dripping alginate solution into a bath containing divalent or trivalent cations, such as calcium chloride. Alginate-recovered chondrocytes (ARC):Chondrocytes that are initially cultured in alginate gel under conditions that allow them to maintain normal phenotype and form a cell-associated matrix (CM) rich in aggrecan, and then recovered along with their CM by dissolution of the alginate gel with a chelating agent.Alkaline phosphatase: A hydrolase enzyme, with an optimum pH of 8.6, distributed throughout the body, with concentrated forms in bone, liver, bile duct, and placenta.Elevated specific activity of the enzyme in cultures of mesenchymal stem cells treated with dexamethasone indicates osteogenic differentiation of the cells.
Authors: Gordana Vunjak-Novakovic PhD, R. Ian Freshney PhD
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4236633504