Three-Dimensional (3D) HepaRG Spheroid Model With Physiologically Relevant Xenobiotic Metabolism Competence and Hepatocyte Functionality for Liver Toxicity Screening
Year: 2017
Type: article
Abstract: doi: 10.1093/toxsci/kfx122 In the MATERIALS AND METHODS section (subsection Assessment of cytotoxicity), we stated the concentrations of all the compounds used: “All the compounds were obtained from Sigma–Aldrich, St. Louis, MO; concentrations used were shown in Supplementary Table 5.” In Supplementary Table 5, we correctly described the exposure range for acetaminophen ranging from 50 mM down to 50 µM. The RESULTS section text also correctly stated, “Acetaminophen showed a TC50 of approximately 6.1 mM (HepaRG spheroids) and approximately 5.8 mM (PHHs) after 24-h exposures, while repeated exposure of 3D HepaRG spheroids produced significantly more potent response with TC50 of approximately 2.9 mM”. However, in Figure 7, which corresponds to the text referenced above, a copy/paste error occurred for one of the curve overlays. In the final version, troglitazone “dose-response image” was copied twice in Photoshop. The correct version of the figure is shown below.
Source: Toxicological Sciences
Language: English
Subfield: Pharmacology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4237711480