Work

The Saccharomyces cerevisiae Genome Contains Functional and Nonfunctional Copies of Transposon Ty1

Year: 1988

Type: article

Abstract: Saccharomyces cerevisiae Ty elements are transposons closely related to retroviruses. The DNA sequence of a functional Ty element (TyH3) is presented. The long terminal repeat sequences are different, suggesting that TyH3 is a recombinant Ty element. A chromosomal Ty element near the LYS2 gene, Ty173, was found to be nonfunctional, even though it has no detectable insertions or deletions. The defect in Ty173 transposition is caused by a missense mutation giving rise to a Leu-to-Ile substitution in the TYB (pol) open reading frame. Several chromosomal Ty elements carry this lesion in their DNA, indicating that nonfunctional Ty elements are common in the yeast genome.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.8.4.1432

Authors: J D Boeke, D Eichinger, D Castrillon, G R Fink

Institutions: Johns Hopkins University, Johns Hopkins Medicine

Language: English

Topic: Chromosomal and Genetic Variations

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: green