Work

Uniform and scalable light-sheets generated by extended focusing

Year: 2014

Type: article

Abstract: Light-sheet fluorescence microscopy (LSFM) affords highly parallelized 3D imaging with optical sectioning capability and minimal light exposure. However, using Gaussian beams for light-sheet generation results in a trade-off between beam waist thickness and the area over which the beam can approximate a light-sheet. Here, we present a novel form of LSFM that uses incoherent extended focusing to produce divergence free light-sheets with near diffraction-limited resolution and uniform intensity distribution along the propagation direction. We demonstrate the imaging performance of the new technique by volumetric imaging of beads, collagen fibers, and melanoma cancer cells with sub-cellular resolution.

Source: Optics Express

DOI: 10.1364/oe.22.026141

Authors: Kevin M. Dean, Reto Fiolka

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,270

Funders: Howard Hughes Medical Institute, Cancer Research Institute, Cancer Prevention and Research Institute of Texas

OpenAlex ID: W4238949196

FWCI: 3.362

Cites: 20

Cited by: 62

Related to: 10