Faculty Opinions recommendation of RNA Docking and Local Translation Regulate Site-Specific Axon Remodeling In Vivo.
Year: 2018
Type: peer-review
Abstract: Nascent proteins can be positioned rapidly at precise subcellular locations by local protein synthesis (LPS) to facilitate localized growth responses.Axon arbor architecture, a major determinant of synaptic connectivity, is shaped by localized growth responses, but it is unknown whether LPS influences these responses in vivo.Using high-resolution live imaging, we examined the spatiotemporal dynamics of RNA and LPS in retinal axons during arborization in vivo.Endogenous RNA tracking reveals that RNA granules dock at sites of branch emergence and invade stabilized branches.Live translation reporter analysis reveals that de novo b-actin hotspots colocalize with docked RNA granules at the bases and tips of new branches.Inhibition of axonal b-actin mRNA translation disrupts arbor dynamics primarily by reducing new branch emergence and leads to impoverished terminal arbors.The results demonstrate a requirement for LPS in building arbor complexity and suggest a key role for pre-synaptic LPS in assembling neural circuits.
Authors: Stephanie Gupton, Shalini Menon
Institution: University of North Carolina at Chapel Hill
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
Awards: MICA: Multi-parametric and super-resolution imaging of amyloidogenic proteins, Development of single molecule techniques for nanoscale imaging of toxic protein species in vitro and in cells., Mechanisms of neurotoxicity of amyloid aggregates., Mechanisms of neurotoxicity of amyloid aggregates., Next generation live super-resolution microscopy: development and application at the Cambridge Advanced Imaging Centre, Axon survival: the role of protein synthesis, Axon navigation in the developing brain., G7823050896, G8039008064
OpenAlex ID: W4239605642