Work

From 3D to 2D: A Review of the Molecular Imprinting of Proteins

Year: 2006

Type: article

Abstract: Molecular imprinting is a generic technology that allows for the introduction of sites of specific molecular affinity into otherwise homogeneous polymeric matrices. Commonly this technique has been shown to be effective when targeting small molecules of molecular weight <1500, while extending the technique to larger molecules such as proteins has proven difficult. A number of key inherent problems in protein imprinting have been identified, including permanent entrapment, poor mass transfer, denaturation, and heterogeneity in binding pocket affinity, which have been addressed using a variety of approaches. This review focuses on protein imprinting in its various forms, ranging from conventional bulk techniques to novel thin film and monolayer surface imprinting approaches.

Source: Biotechnology Progress

DOI: 10.1002/bp060122g

Authors: Nicholas W. Turner, Christopher W. Jeans, Keith R. Brain, Christopher J. Allender, Vladimir Hlady, David W. Britt

Institutions: Utah State University, University of Utah, Cardiff University, Cranfield University

Language: English

Topic: Analytical chemistry methods development

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, U.S. Department of Agriculture, National Heart, Lung, and Blood Institute, National Institute of Biomedical Imaging and Bioengineering

Awards: DIRECT OBSERVATION OF INTERFACIAL PROCESS, NER: Two-Dimensional Sol Gel Protein Imprinting, DIRECT OBSERVATION OF INTERFACIAL PROCESS