Year: 2015
Type: book-chapter
Abstract: We describe two laser microirradiation optical platforms to study: (i) DNA damage responses following selective damage to a single mitotic chromosome arm and (ii) inhibition of cytokinesis resulting from damage to single and/or multiple chromosome tips (pre- sumptive telomeres) of cells in the anaphase stage of mitosis. For studies on DNA damage a 200 femtosecond near-IR laser and a 12 nanosecond 532 nm second harmonic Nd:YVO4 laser were used, and for microirradiation of chromosome tips a 532 nm 12 picosecond second harmonic Nd:YAG laser was used. The DNA damage studies showed that the structure of the alteration was the same for both lasers, and very specific chemical sensing and repair pathways were activated in mitosis. This was evidenced by the accumulation of these proteins at the irradiation sites. Irradiation of the chromosome tips resulted in an inhibition or delay in cytokinesis in a significant percentage of cells. This result suggests that the chromosome tip, the location of telomere, may have a regulatory role in the final stage of cell division.
DOI: 10.1201/b15596-18
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4240921609