Faculty Opinions recommendation of Dynamic assembly of brambleberry mediates nuclear envelope fusion during early development.
Year: 2012
Type: peer-review
Abstract: To accommodate the large cells following zygote formation, early blastomeres employ modified cell divisions.Karyomeres are one such modification, a mitotic intermediate wherein individual chromatin masses are surrounded by nuclear envelope, which then fuse to form a single mononucleus.We identified brambleberry, a maternal-effect zebrafish mutant that disrupts karyomere fusion resulting in formation of multiple micronuclei.brambleberry is a previously unannotated gene homologous to Kar5p, which participates in nuclear fusion in yeast.We demonstrate that Brambleberry is required for pronuclear fusion following fertilization in zebrafish.As karyomeres form, Brambleberry localizes to the nuclear envelope with prominent puncta evident near karyomere-karyomere interfaces corresponding to membrane fusion sites.Our studies identify the first factor acting in karyomere fusion and suggest that specialized proteins are necessary for proper nuclear division in large dividing blastomeres.
Author: Benjamin Feldman
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4241096765