Amplification and Expression of Heterologous Ornithine Decarboxylase in Chinese Hamster Cells
Year: 1988
Type: article
Abstract: We have developed an amplifiable mammalian expression vector based on the enzyme ornithine decarboxylase (ODC). We show greater than 700-fold amplification of this vector in ODC-deficient Chinese hamster ovary cells. A passive coamplified marker, dihydrofolate reductase (dhfr), was amplified and overexpressed 1,000-fold. This ODC vector was a dominant marker in a variety of cell types and displayed at least 300-fold amplification in wild-type Chinese hamster ovary cells.
Source: Molecular and Cellular Biology
DOI: 10.1128/mcb.8.2.764
Authors: T R Chiang, L McConlogue
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1821922395