In Vitro Selection of the Sunflower (Helianthus Annuus L.) Accessions under the Polyethylene Glycol Mediated Drought Stress Conditions
Year: 2016
Type: article
Abstract: Responses of the sunflower accessions to Polyethylene glycol (PEG-6000) mediated drought stress was examined in vitro culture under triplicate completely randomized deign.Three drought treatments i.e.T 1 = Zero (Control), T 2 = -0.35MPa and T 3 = -1.33MPa were developed.Seeds of the accession were grown on half strength MS media and three explants (leaf, stem, coleoptiles) were used for the callus culture.Stress was applied at the time of the media preparation for callus.Fifty petri plates of each accession were prepared and divided into two groups (Group I, Group II) containing 25 plated each.Petri plates of Group I were maintained for callus culture while of Group II were used for regeneration.Data were recorded from these groups on the traits i.e. relative fresh weight (g), proline content (µg), relative membrane leakage (µg), total soluble sugar contents (µg), coleoptiles length (mm), root length (mm), root count, fresh and dry root weight (g) and fresh and dry shoot weight (g).On the basis of the above parameters, the accessions A-23 and HBRS-1 were drought tolerant and 017577 as drought sensitive.In addition, the results of this study illustrate the potential of using in vitro culture for evaluation of drought tolerance at cellular level.
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W4241956040