Work

456. Gene Transfer to Skin Stem Cells by Early Gestational Intra_Amniotic Injection of Lentiviral Vector

Year: 2006

Type: article

Abstract: Early gestational gene transfer may provide developmental opportunities for transduction of accessible stem cell populations that are not available later in development. An example with potential clinical importance is skin_associated stem cells. During development, these cells become inaccessible for gene transfer due to formation of the periderm, stratification of epidermis, and involution of skin accessory structures. To test whether gene transfer to skin stem cells could be achieved early in gestation, and whether gene expression would be maintained long_term, we injected lentiviral vector carrying the green fluorescent protein (GFP) reporter gene into the fetal murine amniotic space from the late head fold/early somite stage (E8) to E18.

Source: Molecular Therapy

DOI: 10.1016/j.ymthe.2006.08.524

Authors: Masayuki Endo, Phil W. Zoltick, William H. Peranteau, Mayumi Ito, George Cotsarelis, Alan W. Flake

Institutions: Children's Hospital of Philadelphia, University of Pennsylvania

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,500