Work

Correction: DNA Replication Timing Is Maintained Genome-Wide in Primary Human Myoblasts Independent of D4Z4 Contraction in FSH Muscular Dystrophy

Year: 2012

Type: erratum

Abstract: Facioscapulohumeral muscular dystrophy (FSHD) is linked to contraction of an array of tandem 3.3-kb repeats (D4Z4) at 4q35.2 from 11-100 copies to 1-10 copies.The extent to which D4Z4 contraction at 4q35.2 affects overall 4q35.2 chromatin organization remains unclear.Because DNA replication timing is highly predictive of long-range chromatin interactions, we generated genome-wide replication-timing profiles for FSHD and control myogenic precursor cells.We compared nonimmortalized myoblasts from four FSHD patients and three control individuals to each other and to a variety of other human cell types.This study also represents the first genome-wide comparison of replication timing profiles in nonimmortalized human cell cultures.Myoblasts from both control and FSHD individuals all shared a myoblast-specific replication profile.In contrast, male and female individuals were readily distinguished by monoallelic differences in replication timing at DXZ4 and other regions across the X chromosome affected by X inactivation.We conclude that replication timing is a robust cell-type specific feature that is unaffected by FSHD-related D4Z4 contraction.

Source: PLoS ONE

DOI: 10.1371/annotation/4fc6a867-640c-4454-ac3b-eb8a386d0ec4