Abstracts of International conference on “Global Environment and Its Sustainability: Implications and Strategies” (GESIS-2010)
Year: 2010
Type: conference-abstract
Abstract: Among the micro algae Botryococcus braunii is one of the organisms known to have high amount of hydrocarbon up to 85% of its dry weight.Therefore throughout the world intensive investigations are being made to utilize this organism for biofuel to meet the demand in future.India is bestowed with different ecosystems and diversities of flora and fauna.The organism, B. braunii is known to occur in oligotrophic water bodies.In the present attempt, the samples of B. braunii were collected from the Southern part of India such as Tamil Nadu, Kerala, Andhra Pradesh and Karnataka.The specimens were collected with a help of 100µm plankton net since the size between 200-800 µm.They were kept in CHU13 medium and brought to the laboratory and maintained at 24+1 o C, 30 µEm -2 s -1 light intensity and 12/12 light dark cycle.Among the samples, a total of 38 isolates were isolated by hand picking using the basal agar medium.They were repeatedly subcultured and inoculated in the basal medium.The contaminants of blue green algae were eliminated with an antibiotic steptomycin sulphate at 2000 ppm under light for 30 min.and transferred to antibiotic free medium and the contaminants are diatoms were eliminated with the medium amended with 5 ppm of germanium di-oxide.Only nine isolates were survived well in the laboratory conditions and the rest of them did not grow and bleached during the study period indicating that they require certain specific environs for their survival.The rest of 9 isolates were studied for their growth by chlorophyll, a, b and total lipid content.Among them two isolates contained high amounts of lipid content.Therefore they were chosen for intensive investigation.One of the interesting observations that we made that the cells in the colonies segregate and grow independently by means of divisions as well as autospore production.The B. braunii (single cell stage) was also studied for its growth and lipid production.Later the cultures were transferred to the open condition and subsequently to race way ponds.A method had been developed successfully to avoid flies in the race way ponds further it also helped to control the contaminants of blue green algae and diatoms.The growth rate of organisms under open race way pond was high than laboratory conditions.The harvested samples of B. braunii under race pond were extracted for total lipid and transesterified into biodiesel and subjected for GC-MS analysis.The prominent fatty acids such as palmitic, oleic and linoleic are present in the fatty acid methyl ester (biodiesel).
Authors: No issue is published in this month owing to the International conference on Global Environment and Its Sustainability Implications and Strategies organized by iSEE The conference proceedings have been published in Volume, Issue, March
Language: English
Field: Energy
Domain: Physical Sciences
Open Access status: hybrid
OpenAlex ID: W4243994641