Work

Benzoate synthesis from glucose or glycerol using engineered Pseudomonas taiwanensis

Year: 2020

Type: preprint

Abstract: Benzoic acid is one of the most commonly used food preservatives, but currently exclusively produced in petrochemical processes. In this study, we describe a bio-based production pathway using an engineered strain of Pseudomonas taiwanensis . In a phenylalanine-overproducing strain, we heterologously expressed bacterial, yeast, and plant genes to achieve production of benzoate via a β-oxidation pathway. Strategic disruption of the native Pseudomonas benzoate degradation pathway further allowed the production of catechol and cis , cis -muconate. Taken together, this work demonstrates new routes for the microbial production of these industrially relevant chemicals from renewable resources.

DOI: 10.22541/au.158981268.89924638

Authors: Maike Otto, Benedikt Wynands, Jan Marienhagen, Lars Blank, Nick Wierckx

Institutions: Forschungszentrum Jülich, RWTH Aachen University

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Responsible consumption and production

Open Access status: gold

Funders: Deutsche Forschungsgemeinschaft, Bundesministerium für Bildung und Forschung

Awards: G1575693250, G449284392, Mikrobielle Biofabriken: NO-STRESS - Die nächste Generation von Plattformorganismen mit außerordentlicher Stresstoleranz (Teilprojekt A), G8252090237

OpenAlex ID: W4244602148

Cites: 17

Cited by: 2

Related to: 10